High-Throughput Raman Process Monitoring of Downstream Protein Purification
Shaun Fraser, Shamus Driver, and Mark S. Kemper
Bruker Optics 40 Manning Road, Manning Park, Billerica, MA 01821, USA
Summary
Raman spectroscopy is emerging as a powerful tool for in-line monitoring and downstream protein analysis. Enabled by HTVStechnology, modern Raman systems deliver up to 30×higher signal, improving sensitivity, speed, and accuracy—even at low laser power. This allows real-time quantification of specific proteins and impurities during chromatographic separation, outperforming traditional UV-vis methods.
By providing actionable insights for process control, Raman supports PAT implementation, enhances product quality, and reduces downstream costs—unlocking significant gains in efficiency and profitability for biopharmaceutical manufacturing.